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Catalog columns resolution

Catalog columns resolution easily

Aug 6th, 2022

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How to catalog columns resolution

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In this video tutorial, we continue our discussion on chromatography by focusing on separation and column resolution. The number of equilibria that a mixture undergoes plays a key role in the effectiveness of separation. A larger difference in partition ratios results in better separation of compounds with fewer equilibria. Conversely, compounds with similar affinities for the stationary phase require more equilibria for separation. The ability to separate compounds is dependent on the ratio of their partition ratios, known as alpha or relative retention, calculated as K Prime 2 divided by K Prime 1. This ratio is always greater than 1.

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