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the guys cook baka mister basics here lets talk about southern blood southern blot is a molecular biology technique used for the detection of specific DNA sequence and the genomic DNA thus technique was introduced by Sir Edwin southern in the Year 1975 lets understand this technique in detail the genomic DNA is first isolated and treated with restriction enzyme the fragments generated by restriction digestion are separated by agarose gel electrophoresis as there are many fragments produced due to restriction digestion a smear is observed on the agarose gel the agarose gel is then treated with an alkali to separate the double-stranded DNA in single-stranded DNA the single-stranded DNA is then transferred on the membrane by placing the membrane on the top of the gel the transfer usually occurs by capillary action once the transfer is complete the membrane is heated in hot air Owen are exposed to UV light for a few seconds this ensures that the DNA is permanently attached with the memb
