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[Music] in this video we will show you how to use stain-free gels in a bio-rad gel imaging system to quickly assess western transfer efficiency and overall transfer quality after transfer of your proteins from the gel to a membrane you may wonder whether the transfer was complete or whether stray air bubbles may have interfered with stain-free gels you can evaluate protein elution from the gel and confirm proper transfer first capture the stain-free gel image after electrophoresis but before transfer using any bio-rad stain-free enabled imaging system after gel activation transfer your proteins to a nitrocellulose or low fluorescence pvdf membrane as usual after completing western transfer retain and image the gel when imaging use the same exposure time as was used to image the gel prior to western transfer the systems auto contrast algorithms will attempt to highlight any signal even if there is very little protein left in the gel after transfer for a fair comparison of the pre and
