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- [Narrator] Western blotting, also called immunoblotting, allows researchers to determine levels of protein expression in a cell or tissue extract through antibody binding to a specific protein of interest. Although this technique is widely used and accepted, problems can occur that lead to suboptimal results, such as low signal or high background, resulting in blots that are difficult to interpret or quantitate. This is the second video in our two-part series on western blotting. The first video, Western Blotting Protocol, provides a comprehensive western blot procedure used in-house by cell-signaling technology scientists to validate our antibodies. This second video provides a guide to help you troubleshoot, suggesting tips to help diagnose problems, and providing solutions to ensure you get the expected results in the shortest amount of time. - Before we begin, its important to note that the single biggest contributing factor to western blot success is the quality of the primary
