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hi Ashley here in this tutorial you will learn the multiple ways you can place a blot on the C digit blot scanner the C digit blot scanner offers flexibility in the way you process your blot for imaging once your blood is ready for substrate to be added you either can choose to wrap the blot in plastic or place the wet blot directly onto the scanner surface remember to always place the blot face down on the imaging surface the block can be placed directly onto the scanner surface either before or after incubation with the chemiluminescent substrate if substrate has not been added to the blot you can add substrate directly to the blot on the scanner surface after the appropriate incubation time for the substrate you can then scan your membrane under normal circumstances the blot will not dry out in the time it takes to acquire an image if you would like to keep the blot wet for an extended period of time you can add a sheet of plastic on top of the blot if you prefer you can wrap your
