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Dot blot is a method for detecting biological samples like proteins or nucleic acids. It is similar to western blotting and southern blotting, but samples are dotted directly onto a membrane. Dot blot is simple and used for various applications such as monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency with biotin, compare the labeled sample to a known biotinylated reference standard through serial dilutions. An example is given using a 10-fold serial dilution for accurate comparison.
