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Dot blot is a quick method for detecting biological samples like proteins or nucleic acids. Unlike western and southern blotting, dot blot involves placing samples directly onto a membrane for detection, rather than transferring them from a gel. Its simplicity allows for various applications, including monitoring labeling efficiency with probes, estimating protein concentration, and comparing antibody performance. For assessing labeling efficiency using biotin, one compares a labeled sample to a known biotinylated reference standard by making serial dilutions of both samples in the same manner, with a 10-fold dilution being effective for a broad range of samples.
