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Dot blot is a method for detecting biological samples such as proteins or nucleic acids, similar to Western and Southern blotting. Samples are directly dotted onto a membrane and probed for detection, used in various applications like estimating protein concentration or comparing antibody performance. To determine labeling efficiency, compare a biotin-labeled sample to a known standard by making serial dilutions of both samples in the same way. A 10-fold dilution is a good starting point for a wide range of samples.
