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Dot blot is a quick and easy method for detecting biological samples like proteins or nucleic acids. It follows a similar principle to western blotting and southern blotting, but samples are dotted directly onto a membrane. Dot blot is used for various applications, such as monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency with biotin, compare the labeled sample to a known biotinylated reference standard through serial dilutions in the same way.
