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[Music] hello this is Sean Taylor field application scientist manager for bio-rad in Canada and Id like to demonstrate now how to perform density metric analysis of gel or blot images using image lab software so what we have before us here is a file that contains a 1 2 dilution series of a protein lysate diluted down from about 60 micrograms loading in 1 2 so 30 15 7.5 all the way down to 6 to the sixth Lane and were going to try and quantify using image lab the density of these more prominent bends that we see here and maybe a less prominent band here so to do density metric analysis with the software you use these toolbars so you have a tool box on the side here and Im only going to demonstrate a couple of the tools in this module just to focus in on density metric analysis so it starts with the image tools and the image tools are used strictly to straighten an image or to rotate or flip the image vertically horizontally the custom rotate tool is quite useful especially if yo
