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This video tutorial discusses the immunodetection step of a western blot, covering the purpose, equipment, supplies, and technique involved. Immunodetection is used to detect specific proteins using polyclonal or monoclonal antibodies. Monoclonal antibodies bind to a single epitope, while polyclonal antibodies bind to several epitopes on a single antigen. To perform immunodetection, you will need a solution, buffer, primary and secondary antibodies, and substrate. After blocking, the primary antibody specific to the target protein is incubated with the membrane, binds to the target protein, and is rinsed. The membrane is then exposed to a specific enzyme-conjugated secondary antibody.
