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This video demonstrates how to attach a column to an NGC chromatography system. Before attaching the column, ensure that the plumbing is connected as per the instruction manual. Prime the pumps with the correct buffer and remove any air from the lines. Use fittings that match the column, disconnect the union and connect the UV sensor inlet to the column outlet. Position the sample injection valve outlet line above the column inlet port and slowly flow buffer. Tighten the fitting until secured, then dry the column.