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all right um so a little were continuing with you know how we can analyze rna secretes so generally there are kind of two pathways we can choose to go down and its kind of dependent on if theres a reference or not so if there is a reference for example we know all the cvss that exist in a species right theres a reference on ensemble theres a reference on ncbi um well i mean what we would do is just clean our reads up and then map them back to the reference and start counting well eventually do that but if we dont have a reference which is probably is still a little bit more common in comparative genomics than what we have to do as a symbol assemble those reads and so its a little bit the idea is is reasonably simple and its basically to just find overlapping reads that share sequence similarity and more or less build a context from that right just a contiguous sequence of reads that overlap with one another and produce what would be a transcript um and so the whole point is
