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The tutorial discusses the use of isobaric tags for relative and absolute quantification, also known as iTRAQ. It involves labeling peptides from different treatments with tags that have increasing mass in the linker group and decreasing mass in the reporter group. This results in labeled amino terminus combinations with specific masses (28+117, 29-116, 30+115, 31+114), enabling them to elute at the same time during liquid chromatography. This approach is used in bottom-up mass spectrometry for data analysis.
