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I would like to talk to you today a little bit about high resolution is not analysis so lets begin by just describing what the technique is and why its so useful so the technique is basically an in-depth analysis of a typical null curve thats produced in a queue PCR reaction so its a post PCR melt analysis method and its used traditionally to determine whether or not you have a specific amplicon in your PCR reaction but if you code can also be analyzed if under the right conditions it can announce it can discriminate the double-stranded amplicon based on sequence length and juicy content which is typically what were looking at when we do a typical null curve for specificity but you can also look at strand complementarity and this is where were this is where we get more into you into looking at single nucleotide polymorphism analysis so these kinds of things or methylation analysis and this is another application to milk River analysis which is what Im going to focus on if it is
