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and everyone quit biochemistry basics here lets talk about resolution of optical microscope in order to understand phenomenon of resolution we must try to understand how the image is formed by the microscope if the light is passed through a small slit it gets deflected the diffraction results in a formation of dark and bright spots it should be noted that as the width of the slit decreases diffraction of light increases now the question is how the diffraction effects ability to see small objects lets consider an example where a slit diffraction Id and produces a diffraction pattern on the screen if we place a convex lens in front of slit then the image of the slit can be seen on the screen instead of diffraction pattern hmm so whats happening here lets see the slits diffracts the light the diffracted light is collected by the convex lens and the lens focuses the diffracted light on one point resulting in an interference pattern which ultimately forms an image now heres the thing