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this video describes how to attach a column to your NGC chromatography system before attaching a column ensure the plumbing is attached as recommended in the instruction manual in addition make sure the pumps are primed with the correct buffer and all lines are purchased of air when attaching the column use only the fittings that match your column begin by disconnecting the union connecting the injection valve and UV detector attach tubing from the UV sensor inlet to the column outlet using the arrow on by our red columns as a directional guide position the sample injection valve outlet line above the column Inlet port then slowly flow buffer from the sample injection valve outlet line into the open Inlet port which is not attached to any tubing continue flowing buffer until the inlet port on top of the column is filled with buffer with the buffer slowly flowing tighten the mail fitting of the sample injection valve outlet line into the column Inlet port until it is secured dry the co