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This tutorial provides step-by-step instructions on how to attach a column to your NGC chromatography system. Before attaching the column, make sure the plumbing is connected as per the manual and the pumps are primed with the correct buffer. Ensure all air is removed from the lines. Only use fittings that match your column. Disconnect the union connecting the injection valve and UV detector. Attach tubing from the UV sensor inlet to the column outlet, following the directional guide on the column. Position the sample injection valve outlet line above the column Inlet port. Slowly flow buffer from the outlet line into the open Inlet port until it is filled. Secure the fitting into the column Inlet port once filled. Dry the column after securing the fitting.