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Untitled - ResearchGate - epa 2025

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The document outlines the standard operating procedures (SOP) for extracting holocellulose and Į-cellulose from dried plant tissues at WED's Integrated Stable Isotope Research Facility. It details the necessary equipment, chemicals, and step-by-step processes involved in sample preparation, extraction, boiling, bleaching, and quality control measures. The objective is to provide clear instructions for researchers to obtain pure holocellulose or Į-cellulose for isotopic analysis while ensuring safety and quality assurance throughout the procedure.

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Click ‘Get Form’ to open it in the editor.
Begin by reviewing the Table of Contents to familiarize yourself with the sections, such as Introduction, Objective Statement, and Sample Preparation.
Fill out your name, date, and project information at the top of each IDSheet provided in the document.
For Sample Preparation, follow the detailed instructions for grinding and preparing extraction bags. Ensure you cut and seal the ANKOM filter paper correctly.
As you proceed to Bag Clipping, ensure each packet is uniquely identified according to the IDSheet. This will help maintain organization throughout your process.
When loading samples into packets, double-check that all materials are placed correctly and that each pocket is sealed before submission.
After completing all steps, copy your final IDSheets for records and keep originals safe for reference during processing.

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Partnering Internationally. EPA is engaged in a variety of international activities to advance climate change science, monitor the environment, and promote activities that reduce greenhouse gas emissions. EPA establishes partnerships, provides leadership, and shares technical expertise to support these activities.