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Acid-fast staining (Figure 5-39) is used to screen for the presence of Mycobacterium, Nocardia, and Legionella species in body tissues and fluids. Some oocysts, such as Cryptosporidium, can be detected with the acid-fast stain.
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
An acid-fast bacteria (AFB) culture is done to find out if you have tuberculosis (TB) or another mycobacterial infection. Besides TB, the other main mycobacterial infections are leprosy and a TB-like disease that affects people with HIV/AIDS.
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
Acid-fast bacteria are gram-positive in terms of structure because they contain peptidoglycan in the cell wall. However, they stain poorly with Gram stain procedure, appearing weakly Gram-positive.

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TUBERCULOSIS, LEPROSY AND OTHER DISEASES CAUSED BY ACID-FAST BACTERIA. Science.
Acid- Fast Bacilli (AFB) smear and culture are two separate tests always performed together at the MSPHL, Tuberculosis (TB) Unit. AFB smear refers to the microscopic examination of a fluorochrome stain of a clinical specimen.
If your AFB smear was positive, it means you probably have TB or other infection, but an AFB culture is needed confirm the diagnosis. Culture results can take several weeks, so your provider may decide to treat your infection in the meantime.
Bacteria displaying acid fastness include: Genus Mycobacterium M. leprae, M. tuberculosis, M.These include: Bacterial endospores. Head of sperm. Cryptosporidium parvum. Isospora belli. Cyclospora cayetanensis. Taenia saginata eggs. Hydatid cysts. Sarcocystis.
A positive test result from the acid-fast stain confirms the patient has TB. In other types of acid-fast bacteria such as Nocardia, only certain parts of each cell retain the dye, such as the wall of the cell. A positive test result from a partial or modified acid-fast stain identifies these types of infections.

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Laboratory Examination: Acid-Fast Bacilli (AFB) Smears (1)

Detection of acid-fast bacilli (AFB) in stained and acid-washed smears examined microscopically may provide the initial bacteriologic evidence of the presence of mycobacteria in a clinical specimen. Smear examination is a quick procedure; results should be available within 24 hours of specimen collection.

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Laboratory Examination: Acid-Fast Bacilli (AFB) Smears (1)

Detection of acid-fast bacilli (AFB) in stained and acid-washed smears examined microscopically may provide the initial bacteriologic evidence of the presence of mycobacteria in a clinical specimen. Smear examination is a quick procedure; results should be available within 24 hours of specimen collection.

Learn more
Acid-Fast Bacilli (AFB) Smear and Culture

Acid- Fast Bacilli (AFB) smear and culture are two separate tests always performed together at the MSPHL, Tuberculosis (TB) Unit. AFB smear refers to the microscopic examination of a fluorochrome stain of a clinical specimen.

Learn more

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