Virology: COVID-19 Testing 2025

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Viral tests, including nucleic acid amplification tests (NAATs) and PCR tests, as well as antigen tests, are used as diagnostic tests to detect current infection with SARS-CoV-2, determine the need for prevention measures, and inform a persons medical care.
Antigen tests* are rapid tests that usually produce results in 15-30 minutes. Positive results are accurate and reliable. However, in general, antigen tests are less likely to detect the virus than NAAT tests, especially when symptoms are not present. Therefore, a single negative antigen test cannot rule out infection.
It is a nonsegmented and RNA virus ranging from 26 to 32 kb. Coronavirus comes under the order-nidovirus, family-coronaviridae, subfamily-coronavineae which were further divided into , , and genus. Among these, and genus of Coronavirus mainly affect the human population.
There are two types of COVID-19 tests: the polymerase chain reaction (PCR) test and the antigen test, known as the rapid test. Both tests require a sample from the patient, usually a nasal swab.
Molecular tests, such as polymerase chain reaction (PCR) and other nucleic acid amplification tests (NAATs) tests, which detect genetic material called RNA from the virus. Antigen tests, often referred to as rapid tests or, for some, at-home or self tests, which detect proteins called antigens from the virus.

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The PCR testing then detects this DNA. Hence the name RT-PCR. These are also known as the diagnostic test and nucleic acid amplification test (NAAT). How the sample is taken- A nasal or throat swab sample is taken.

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