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Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species).
Inadequate sterilization is a significant cause of exogenously acquired infections in health care. Sterilization failures can and do occur. Without proper monitoring, these failures may get overlooked, resulting in serious consequences for the practice.
Types of Sterilization Steam Sterilization. Dry Heat Sterilization. Ethylene Oxide (EtO) Sterilization.
The effectiveness and proper performance of a sterilizer must be confirmed through a combination of three types of monitoring: physical or mechanical, chemical and biological.
Sterilization indicators, such as spore strips and indicator tape, enable routine monitoring, qualification,and load monitoring of the steam sterilization process. They indicate whether the conditions during a steam autoclave cycle were adequate to achieve a defined level of microbial inactivation.
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Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species).
Resterilized paper/plastic pouches could maintain sterility after at least up to 6 times of steam sterilization and up to 6 months of storage in a closed environment.
Steam chemical indicators (e.g., autoclave tape, steam indicator strips) should be used in each autoclave load. These indicators change color in the presence of steam and when placed on the surface of items are a quick visible indicator that the items have been autoclaved.
Sterilization destroys all microorganisms on the surface of an article or in a fluid to prevent disease transmission associated with the use of that item.
Autoclave indicators are used to validate that the autoclave is working properly.

autoclave spore test log sheet