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The phylogeny and evolution of deoxyribonuclease II - University of

The phylogeny and evolution of deoxyribonuclease II - University of 2025

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The document discusses the phylogeny and evolution of deoxyribonuclease II (DNase II), an enzyme crucial for lysosomal DNA degradation in eukaryotes. It explores the enzyme's distribution across various organisms, suggesting that DNase II likely originated early in eukaryotic evolution, possibly linked to phagocytosis. The study highlights its presence in metazoans and certain protists while noting its absence in others, such as plants and fungi, which may indicate secondary losses due to changes in feeding strategies. The authors emphasize the need for further genomic data to clarify the evolutionary history of DNase II.

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What organisms are involved in deoxyribonuclease?

They are produced by a variety of Gram-negative pathogenic bacteria, among them E. coli, Shigella dysenteriae, Campylobacter sp., Helicobacter sp., and S. enterica.

What organisms are positive for the DNase test?

Deoxyribonuclease (DNAse) is an enzyme for degrading DNA to fragmentation by catalyzing the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. DNA is degraded during cell death that accompanies a number of diseases.

What does deoxyribonuclease break DNA into?

DNase I cleaves DNA to form two oligonucleotide-end products with 5-phospho and 3-hydroxy ends, while DNase II cleaves DNA to form two oligonucleotide-end products with 5-hydroxy and 3-phospho ends. DNase I is produced mainly by organs of the digestive system, such as the pancreas and salivary parotid glands.

What are the different types of deoxyribonuclease?

It breaks the phosphodiester bond formed between two nucleotides of dsDNA or ssDNA, by cleaving either at/on ends or between the nucleic acid strands. The enzyme is encoded by two different DNase genesdifferent in length and number of exonsand thus, is of two types: DNase I and DNase II.

Who discovered deoxyribonuclease?

However, the description of DNA actually began 135 years ago with its discovery by Friedrich Miescher, a much less known man who isolated the hereditary material in 1869.

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What produces deoxyribonuclease?

DNase I is produced mainly by organs of the digestive system, such as the pancreas and salivary parotid glands.

What bacteria produces deoxyribonuclease?

Various hemolytic streptococci, hemolytic staphylococci, and micrococci were tested for production of deoxyribonuclease (DNase). In general, beta-hemolytic pathogenic streptococci of human origin produced highest concentrations of DNase. However, some beta-hemolytic bovine strains were also highly productive.

The phylogeny and evolution of deoxyribonuclease II - University of 2025

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What organisms are involved in deoxyribonuclease?

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