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BOD Analysis: Basics and Particulars - Wisconsin Department of - dnr wi

BOD Analysis: Basics and Particulars - Wisconsin Department of - dnr wi 2025

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The document provides a comprehensive guide on Biochemical Oxygen Demand (BOD) analysis, detailing its significance, methodology, quality control measures, troubleshooting common issues, and necessary documentation for compliance. It emphasizes the importance of BOD testing in assessing waste strength and outlines best practices for calibration, sample handling, and quality assurance to ensure accurate results.

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What are the requirements for the BOD pod test?

What to Expect All testing is done by appointment. 30-minute appointment. Special clothing is necessary. Dont eat or exercise 4-5 hours prior to your test. Dont drink coffee 4-5 hours prior to your test. Dont smoke or drink alcohol 2 hours prior to your test.

What is the standard method of BOD analysis?

The method consists of filling with sample, to overflowing, an airtight bottle of the specified size and incubating it at the specified temperature for 5 d. Dissolved oxygen is measured initially and after incubation, and the BOD is computed from the difference between initial and final DO.

What is the analysis of BOD?

BOD provides a measure of the impact of a waste (water) on the oxygen content in a receiving water body. Wastes (organic matter, food) are broken down (decomposed, oxidized, metabolized) by microbial organisms (aerobic bacteria), frequently referred to as bugs, who require oxygen for this monumental effort.

What is the 2 1 rule for BOD?

The goal is to have at least one sample deplete by more than 2.0 mg/L oxygen and still have at least 1.0 mg/L oxygen remaining. This is known as the 2:1 rule. If more than one dilution bottle has acceptable depletion, the final results for each bottle are averaged together to give one reported result.

What are the standards for BOD?

Typical values. Most pristine rivers will have a 5-day carbonaceous BOD below 1 mg/L. Moderately polluted rivers may have a BOD value in the range of 2 to 8 mg/L. Rivers may be considered severely polluted when BOD values exceed 8 mg/L.

What are the requirements for BOD?

At a minimum, there should be at least a 2.0 mg/L DO change between the initial and the final reading. There should also be a residual DO of at least 1.0 mg/L. The blank, seed determination, and glucose-glutamic acid standard should be run every time a BOD analysis is performed.

What is the procedure for BOD?

BOD measurement requires taking two samples at each site. One is tested immediately for dissolved oxygen, and the second is incubated in the dark at 20 C for 5 days and then tested for the amount of dissolved oxygen remaining.

What is the 5 day BOD test?

The standard oxidation (or incubation) test period for BOD is 5 days at 20 degrees Celsius (C) (BOD5). The BOD5 value has been used and reported for many applications, most commonly to indicate the effects of sewage and other organic wastes on dissolved oxygen in surface waters (see TECHNICAL NOTE).

BOD Analysis: Basics and Particulars - Wisconsin Department of - dnr wi 2025

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