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Major mediator of apoptosis is a family of proteins known as caspases. There are mainly fourteen types of caspases but out of them only ten caspasese have got essential role in controlling the process of apoptosis.
Inflammatory caspases do not function in apoptosis but are rather involved in inflammatory cytokine signaling and other types of cell death such as pyroptosis.
The anti-apoptotic members of this family, such as Bcl-2 and Bcl-XL, prevent apoptosis either by sequestering proforms of death-driving cysteine proteases called caspases (a complex called the apoptosome) or by preventing the release of mitochondrial apoptogenic factors such as cytochrome c and AIF (apoptosis-inducing
THE FLOW CYTOMETRY CASPASE (FCC) ASSAY The method utilizes fluorogenic caspase substrates that detect caspase activation within individual target cells. The substrates are composed of two covalently linked fluorophores that are cleaved by caspases.
The Caspases are specific proteases that are active in apoptosis to cleave intracellular proteins. They are called caspases because they are cysteine aspartate proteases that have cysteine at catalytic site and cleave target proteins after aspartate residue.
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In so-called type I cells, caspase-8 initiates apoptosis directly by cleaving and thereby activating executioner caspases. In type II cells, caspase-8 must first activate the intrinsic apoptotic pathway (discussed below) to induce efficient cell death (Samraj et al.
Caspases, a unique family of cysteine proteases, execute programmed cell death (apoptosis). Caspases exist as inactive zymogens in cells and undergo a cascade of catalytic activation at the onset of apoptosis. The activated caspases are subject to inhibition by the inhibitor-of-apoptosis (IAP) family of proteins.
Caspases were originally identified as important mediators of inflammatory response and apoptosis. Recent discoveries, however, have unveiled their roles in mediating and suppressing two regulated forms of necrotic cell death, respectively termed pyroptosis and necroptosis.

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