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What are the applications of FACS in immunology?

It can be used for Immunophenotyping, DNA cell cycle/ tumor ploidy, Membrane potential, Ion flux, Cell viability, Intracellular protein staining, pH changes, Cell tracking and proliferation, Sorting, Redox state, Chromatin structure, Total protein, Lipids, Surface charge, Membrane fusion/ run-over, Enzyme activity,

What is flow cytometry used for immunology?

Why is flow cytometry used in immunology? Flow cytometry has been conventionally associated with the use of monoclonal antibodies to identify immuno-competent cells, to quantify changes in expression of surface determinants, and to separate cell population subsets before testing their functional characteristics.

What is the difference between FACS and flow cytometry?

The terms flow cytometry and FACS are often used interchangeably. However, this is not accurate. Namely, FACS is a subtype of flow cytometry that allows cells to be sorted and retained rather than simply counted, analyzed, and disposed of.

How much does a flow cytometer cost?

The flow cytometer is a complex, expensive device that must simultaneously run multiple tasks. Even used, outdated machines can run upwards of $35,000. The average used cytometer equipped with standard features will cost anywhere between $80,000 and $150,000.

Why is flow cytometry expensive?

Although flow cytometry is the gold standard for accurate and automated measurement of CD4 T lymphocytes, the technique is expensive and requires sophisticated equipment as well as trained personnel to perform it.

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