Drug Testing LaboratoriesUS Department of Transportation 2025

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Guidance: Driver violation records will be available in the Clearinghouse for five years from the date of the violation determination, or until the violation is resolved through the successful completion of the return-to-duty (RTD) process and follow-up testing plan, whichever is later. See 49 CFR 382.719.
It is essential to note that if you refuse to take a drug test or if you fail a drug test, it is considered a violation of DOT regulations, and your CDL may be suspended or revoked as well.
But commercial truck drivers are using illegal drugs at an alarming rate. DOT reports that 69,668 truck drivers failed their drug test in 2022, up 18% over the year before, which was double the 9% increase the year before that. DOT only recognizes a urinalysis or saliva testing to detect a truck drivers drug use.
For questions, please contact DOTs Office of Drug Alcohol Policy Compliance at 202-366-DRUG (3784) or visit our website at .transportation.gov/odapc.
Saliva Testing Now Allowed The DOT published a final rule in the Federal Register on May 2, 2023, which was effective on June 1, 2023. This rule allows employers to use saliva or oral fluid tests instead of tests to complete DOT drug testing. Previously, only five-panel tests were allowed.
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When you work for the DOT and have your CDL, you have to pass drug and alcohol tests at regular and random intervals. This is to ensure that youre staying sober on the job for both your own and others safety. If you fail one of these tests, your CDL will be revoked.
What Type of Drug Tests Are Used? Various methods are used to thoroughly check for substance use. One important method is the Department of Transportation (DOT) drug test, which typically involves examining samples for prohibited substances.
The length of time a failed drug screen stays on a record depends on the employer and the type of record. Records from a failed DOT drug test remain in the Drug and Alcohol Clearinghouse Database for five years or until a person resolves the violation through the return-to-duty process and follow-up testing.

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