Dental spore test log sheet 2025

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Spore test results: A passed (negative) spore test means the sterilizer is working properly. A failed (positive) spore test means the sterilizer is not working properly. Keep copies of the spore test results for three years with a minimum of one year (365 days) on site.
NOTE: All autoclaves should be validated by spore testing: Monthly for healthcare use, weekly or daily for dental depending on the frequency of autoclaving.
In-office spore testing procedure Select 2 ampoules from the same lot number. Place the test ampoule in the autoclave per the IFU. Run the autoclave using the correct cycle and parameters. Remove the ampoule from the autoclave.
Assuming the autoclave is set to the correct temperature and time, here are a few things to check: The sterilization time was not long enough. The chamber drain strainer might be clogged. If you are running a pre-vacuum cycle make sure the vacuum pulses are working by looking at the printer tape or cloud data.
Steam and low temperature sterilizers (e.g., hydrogen peroxide gas plasma, peracetic acid) should be monitored at least weekly with the appropriate commercial preparation of spores.

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A spore test should be used at least weekly to monitor sterilizers, and should use a matching control (i.e., biological indicator and control from same lot number). Follow the manufacturers directions for how to place the biological indicator in the sterilizer.
Spore test at least once a week California Code of Regulations states: Proper functioning of the sterilization cycle of all sterilization devices shall be verified at least weekly through the use of a biological indicator (such as a spore test). Test results shall be documented and maintained for 12 months.

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