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many biological molecules recognize other specific biomolecules and bind to them with high affinity as part of their normal function for example hormones are recognized by specific receptor proteins antibodies recognize their respective antigens enzymes recognize their respective substrates this ability to recognize a partner molecule is harnessed in affinity chromatography for instance if we conjugate that is attach an enzyme onto the surface of sepharose beads we generate a stationary phase that can isolate the substrate of that enzyme from a complex mixture one pair of biomolecules that binds to each other with high specificity are the enzyme glutathione s transfer is gst and its substrate glutathione this pair is involved in with the removal of toxins from the body glutathione is a dry peptide with sulfhydryl group shown here in red the importance of the sulfur hydral group is reflected in the abbreviation we use for glutathione which is g sh glutathione s transferases are enzymes
