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The document is a registration form and materials transfer agreement for the NIH Tetramer Facility. It outlines the requirements for registrants, including submission of a signed registration form, a biographical sketch, and shipping account information. The agreement specifies the conditions under which tetramers and related materials can be used, emphasizing non-commercial research purposes, compliance with safety standards, and restrictions on human use. It also details liability disclaimers, acknowledgment of source in publications, and reporting obligations to the NIH Tetramer Facility.

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What is tetramer and its structure?

Tetramer: A structure composed of four identical smaller units, or four very similar smaller units. The units may be associated by covalent bonds or by noncovalent forces. This tetrapeptide is Gly-Gly-Gly-Gly, a tetramer composed of four identical amino acid residues (each is a glycine).

How does a tetramer work?

Tetramer analysis is a technique that uses MHC molecules to detect T-cells that recognize peptides or antigens from infected cells or cancerous cells. A tetramer has four MHC molecules attached to a streptavidin-coated bead. T-cells that recognize the peptide in the four MHC molecules will bind to the tetramer.

What are tetramer CD8 T cells?

Tetramer stains usually analyze cytotoxic T lymphocyte (CTL) populations. CTLs are also called CD8+ T-cells, because they have CD8 co-receptors that bind to MHC class I molecules.

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What do tetramers do?

The tetramers used in the assay are made up of four major histocompatibility complex (MHC) molecules, which are found on the surface of most cells in the body.... Tetramer assaySynonymsTetramer stainPurposeuses tetrameric proteins to detect and quantify T cells

What are MHC tetramers used for?

MHC tetramers are used for the analyses of T cell immunity, as these reagents allow accurate enumeration and efficient immunomagnetic sorting of antigen-specific T cells, regardless of the T cell's functional capacity.

What is tetramer staining?

A tetramer assay (also known as a tetramer stain) is a procedure that uses tetrameric proteins to detect and quantify T cells that are specific for a given antigen within a blood sample.

How is a tetramer made?

In the presence of streptavidin which has four biotin binding sites per molecule, four MHC monomers are joined together to form a tetramer.

How are tetramers formed?

11.19. Major histocompatibility complex (MHC) tetramers are formed by first refolding MHCs in the presence of high concentrations of the desired antigenic peptide, followed by biotinylation of the carboxy-terminus of one chain of the MHC molecule.

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