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How should sterilization indicators be stored? A. In a cool, dry area.
Sterilization indicators, such as spore strips and indicator tape, enable routine monitoring, qualification,and load monitoring of the steam sterilization process. They indicate whether the conditions during a steam autoclave cycle were adequate to achieve a defined level of microbial inactivation.
8 Sterilizer maintenance and repairs should be documented in a paper or electronic record keeping system. 9 Sterilization records should be kept for seven years. facilities: Safety and effectiveness (ANSI/AAMI ST41) document recommends the use of a BI PCD in every other ethylene oxide sterilization cycle.
Table-top Steam Sterilizers. The purpose of this document is to record process parameters for steam sterilization in community health care settings. This will assist with tracking of medical devices used on clients/patients/residents in the event of a recall or follow-up investigation.
For each sterilization cycle, record the type of sterilizer and cycle used; the load identification number; the load contents; the exposure parameters (e.g., time and temperature); the operator's name or initials; and the results of mechanical, chemical, and biological monitoring.
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The effectiveness and proper performance of a sterilizer must be confirmed through a combination of three types of monitoring: physical or mechanical, chemical and biological. Use of one type of indicator does NOT replace the need to use the other two types.
How often should biological monitoring (spore testing) be done? A spore test should be used on each sterilizer at least weekly. Users should follow the manufacturer's directions for how to place the biological indicator in the sterilizer. A spore test should also be used for every load with an implantable device.
Completed autoclave use logs must be retained by laboratory staff for 3 years. Autoclave record keeping will be confirmed during yearly laboratory safety surveys.
Specific temperatures must be obtained to ensure the microbicidal activity. The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F). These temperatures (and other high temperatures)830 must be maintained for a minimal time to kill microorganisms.
Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species).

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