3, mF o form a ? 2 - ? - ? 2 pulse sequence T he fi rst ? 2 2025

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A 180 RF pulse can rephase spins and reverse static field inhomogeneities. After a 90 RF pulse, spins dephase and transverse magnetization decreases. If we apply a 180 RF pulse, spins rephase and transverse magnetization reappears.
Thus, a 90 pulse width is the amount of time the pulse of energy is applied to the particular sample in order to flip all the spins into the X-Y plane, i.e., the condition shown in Figure 2A. The 90 pulse width for proton NMR experiments is set to about 8-13 s on most instruments.
To become an NMR ninja, you must learn how to select a pulse width that changes the angle by 90. This is because a flip angle of 90 produces the maximum measurable signal, which is essential for great NMR spectra. The most important part is choosing the length of time for an RF pulse that produces the maximum signal.
In Fourier transform NMR spectroscopy and imaging, a pulse sequence describes a series of radio frequency pulses applied to the sample, such that the free induction decay is related to the characteristic frequencies of the desired signals.
In the pulse NMR experiment, high-power rf pulses are applied to a sample for rotating the equilibrium longitudinal magnetization, and, subsequently, the free-induction decay (FID) of the transverse component is detected as a time-dependent function.
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In this chapter you will learn a few of the ways that a time domain signal can be created. Three methods are presented here, but there are an infinite number of possibilities. These methods are called pulse sequences. A pulse sequence is a set of RF pulses applied to a sample to produce a specific form of NMR signal.

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