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Method for site-specific integration of nucleic acids - Google Books

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The document US005916804A describes a patent for a method of site-specific integration of nucleic acids, developed by Frederic D. Bushman and assigned to The Salk Institute for Biological Studies. It details the creation of chimeric proteins that consist of a DNA-binding domain and an integrase domain, allowing for targeted integration of donor nucleic acids into specific sites on target nucleic acids. The invention aims to improve gene therapy techniques by providing methods and constructs that facilitate precise genetic modifications while minimizing risks associated with random integrations.

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What is the method of quantification of nucleic acids?

UV Spectrophotometry using absorbance at 260 nm Absorbance has been the method of choice for routine quantification of DNA and RNA since decades. It is simple and convenient to use as no further sample treatment (other than DNA extraction) or reaction with other substances is required.

What are the different methods used to purify and quantify amounts of nucleic acids?

There are several experimental techniques to detect and quantify nucleic acids in your sample. These include PCR, RT PCR, sequencing and blotting. There are also techniques such as EMSA, footprinting, ChIP and probing that detect inter-molecular interactions of nucleic acids.

What are the experimental methods to study protein nucleic acid interactions?

DNA sequencing. DNA sequencing is the process of determining the nucleic acid sequence the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine.

What are the methods of detection of nucleic acids?

Two common methods are immunoblotting (also called Western blotting) and immunoprecipitation. Western blotting (Figure 3.31) is another variation of Southern blotting. Proteins in cell extracts are first separated ing to size by gel electrophoresis.

What are the methods of nucleic acid extraction?

Nucleic acid is often purified from cells as part of an ever-growing array of molecular biology methods, including sequencing and gene editing. Before they are used in downstream applications, nucleic acids are detected and quantitated using UV or fluorescence spectrophotometry.

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What are the methods of detection and quantification of nucleic acids?

Fluorescence-based methods. The use of fluorescent tags is widespread in the studies of proteinnucleic acid interactions for imaging and structural/functional studies on both single-molecule and population levels.

What methods are there for checking the quality of nucleic acids?

Overview of Nucleic Acid Quantification Methods The most common methods include UV-Vis spectrophotometry, fluorimetry, real-time quantitative PCR (qPCR), gel electrophoresis and capillary electrophoresis.

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