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What is protein biotechnology?

Protein production is the biotechnological process of generating a specific protein. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene.

How much protein do you need for proteomics?

How much material do I need, and how pure does the sample need to be? We need a minimum of 0-15 ug total protein of a more pure (80% based on a coomassie standed SPD-PAGE) sample or 20-3 ug total protein of a less pure (50% based on coomassie stained SDS-PAGE) sample to maximize the chances of success.

How much protein is needed for mass spectrometry?

For a label-free quantitative proteomics experiment typically 20 g of total protein per sample are required. This amount corresponds to approximately 300,000 cells. For a phosphoproteomic analysis about 500 g total protein per sample are needed.

How much protein do you need for phosphoproteomics?

Sample Preparation For example, large-scale phosphoproteomic experiments performed on mammalian cells often require 3 to 5 mg of protein extracts in order to identify more than 10,000 distinct phosphorylation sites.

How many proteins are in proteomics?

Current bottom-up quantitative proteomics methods based on MS/MS sequencing of peptides usually only monitor a small proportion of the proteome of higher eukaryotic cells. Typically 20003000 proteins are observed,1-4 with a maximum so far of 5,111.