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A sequence of 245 base-pairs (oriC) in the replication origin of the Escherichia coli K-12 chromosome has been shown to provide all the information essential for initiation of bidirectional replication.
coli). HTH helix-turn-helix domain, DUE DNA unwinding element, IHF integration host factor. Most bacterial chromosomes are circular and contain a single origin of chromosomal replication (oriC).
In E. coli a single round of genome replication lasts about 40 min and it must be accomplished about 20 min before cell division. To achieve such fast growth rates bacteria perform multiple replication rounds simultaneously.
How many numbers of replicon is found in E. coli? Explanation: E. coli is monorepliconic and have single replicon while eukaryotic cells contain many replication origins on a single chromosome and called multirepliconic.
The replication origin of the E. coli K-12 chromosome has been isolated as autonomously replicating molecules(oriC plasmid), and the DNA region essential for replicating function(oriC) has been localized to a sequence of 232-245 base-pairs(bp) by deletion analysis.
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During DNA replication, the complementary strands of DNA must unwind completely to allow the synthesis of a new strand on each template. The number of helical turns = number of base pairs/number of base pairs per helical turn. Thus, 4.64 x 106/10 = 4.64 x 105 turns must be unwound.

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