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What are the first 5 ) two nucleotides of an intron?

Introns always have two distinct nucleotides at either end. At the 5 end the DNA nucleotides are GT [GU in the premessenger RNA (pre-mRNA)]; at the 3 end they are AG. These nucleotides are part of the splicing sites.

Does intron length matter?

Specifying the maximum intron length helps because it limits the search space for the other end of a read when it is being aligned to the genome.

How short can an intron be?

Paramecium tetraurelia has the shortest known introns as its standard intron length. Sequenced introns vary between 20 and 33 nucleotides in length.

Do all introns start with GT?

Analysis of cDNA-confirmed introns in humans shows that the vast majority of introns begin with the dinucleotide GT and end with the dinucleotide AG. This GT-AG intron class constitutes 99.24% of human introns (68).

What is the range of intron length?

It is generally considered that sequences with length 40 bp cannot form a complete intron and a mature intron should not be shorter than 80 bp. Account of this, introns are divided into nine groups by length. The length range and standard length of each intron group are as shown in Table 2.

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