Fix detail in PAP

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Aug 6th, 2022
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How to fix detail in PAP

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okay so Iamp;#39;m going to show you how to prepare yet what you do is you have the material on a site usually is about two millimeters in diameter you pick up a slide with one hand and the label flight site is facing away then you pick up the spreaders like place the edge of the spreaders light thatamp;#39;s closer to you just gently down on a slight perpendicular to the basis light and then use the weight of the slide to spread partner material after which it very gently just slide it down and immediately 6:00 in 95 percent alcohol so the ideal shape of the smear should be convex by convex lanta form shape and this is optimal for assessing psychologically because the cells will be evenly spread out

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Typically, your doctor will use the Loop Electrosurgical Excision Procedure (LEEP), or perform a cold knife cone biopsy as a minor surgical procedure. Be sure to always bring up any questions you may about you Pap test with your gynecologist.
Out of which, 95% ethanol is the commonly used fixative. Hence, the conventional method for fixation of Pap smear is to fix the Pap smear immediately in 95% ethyl alcohol after preparing the smear.
A procedure in which a small brush is used to gently remove cells from the surface of the cervix and the area around it so they can be checked under a microscope for cervical cancer or cell changes that may lead to cervical cancer.
Ethyl alcohol is the fixative specifically recommended for cytological preparations. Fixation coarsens the cell structures and sharpens nuclear chromatin pattern and its details.
Pap staining procedure Mayer Hematoxylin and Gills hematoxylin is preferred. In regressive method the smear is over stained with nuclear stain with a non-acidified hematoxylin. The excess stain is removed with dilute HCL acid solution. Harris hematoxylin is preferred for regressive method.
The smear(s) should be immediately fixed with spray fixative or fixed in 95% ethyl alcohol for 10 minutes. Allow the slides to completely air dry before shipping. Scrape additional areas of the vagina that appear abnormal and spread and fix as noted above.
Smears must be fixed immediately before they dry. A 95% solution of ethyl alcohol or spray fixative is used to fix the slide. The slides should be labeled in pencil with the patients name AND date of birth and a properly completed Pap smear requisition should accompany the slide.
An abnormal Pap test result rarely means cancer is present, especially in someone who has regular, annual Pap tests. An abnormal result means there are cells in the cervix that look unusual, which can be caused by yeast or bacterial infection, irritation, benign growths, hormonal changes, or pre-cancer.

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