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Dot blot is a simple and efficient method for detecting biological samples like proteins and nucleic acids. It operates similarly to western and southern blotting but involves directly dotting samples onto a membrane instead of transferring from a gel. This technique supports various applications, including monitoring labeling efficiency with probes, estimating protein concentration, and comparing different antibodies' effectiveness. For example, to assess labeling efficiency using a biotinylated sample, one would compare it to a known biotinylated reference standard, creating serial dilutions of both the test sample and the reference in the same manner, typically starting with a 10-fold serial dilution for a broad range of samples.
