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host celled protein Eliza and HCP antibody coverage analysis and Eliza or enzyme-linked immunosorbent assay test kit uses specifically designed and reactive antibodies to detect and quantify host cell protein or HCP impurities found in bio pharmaceutical drug substances the identification and monitoring of HCPs is critical to ensure purification process consistency and final drug substance purity the capture and detection antibodies found in most Eliza kits usually only test for one or a few proteins at a time but the antibodies found in the cygnus HCP iliacus are different theyamp;#39;re broadly reactive and can measure hundreds of different HCP impurities in a single assay but before we get too far ahead letamp;#39;s walk through how this process actually works the assay itself only consists of two main steps first add anti HCP antibodies conjugated with HRP enzyme and a test sample containing HCPs or HCP standards to the well coated with anti HCP antibodies cover and place the pla