Dd 686 bacteriological form 2025

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Wear gloves and eye protection when collecting samples. Rinse the bottle and cap three times with sample water and fill the bottle to within one to two inches from the top. Place the sample into a cooler with ice for immediate delivery or shipment to the laboratory. Sterile 125 or 150 mL plastic bottles must be used.
It involves the collection of water samples from various sources, followed by laboratory analysis to detect the presence of harmful microorganisms, particularly bacteria. The primary goal is to identify potential pathogens such as Escherichia coli (E.
Use a cotton swab to collect bacteria samples (e.g., door handle, unwashed or clean hands, your mouth before and after brushing your teeth, kitchen sink). Make sure to use one swab per surface. 10. After swabbing a surface, softly draw a squiggly line across the agar and close the lid / recover with plastic wrap.
The laboratory procedure involves making serial dilutions of the sample (1:10, 1:100, 1:1000, etc.) in sterile water and cultivating these on nutrient agar in a dish that is sealed and incubated. Typical media include plate count agar for a general count or MacConkey agar to count Gram-negative bacteria such as E.
There are several methods to test for bacteria, but plate count is the most reliable. A water sample and a nutrient agar is placed and sealed in clear dish. Agar contains substances that can help determine the type and amount of bacteria in the sample.