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XF Glycolysis Stress Test Kit User Manual - Seahorse Bioscience 2025

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The XF Glycolysis Stress Test Kit User Manual provides detailed instructions for using the kit with the XF24 Analyzer to assess cellular glycolytic function. It outlines the assay's principles, including the measurement of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) to evaluate glycolysis, glycolytic capacity, and reserve. The manual includes preparation guidelines for assay components, optimization assays for cell seeding density and oligomycin concentration, and step-by-step procedures for running the test and analyzing data. This kit is intended for research use only.

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How does Seahorse XF work?

Seahorse Bioscience Extracellular Flux Analyzer The Seahorse XF-96 measures the rate of change of analytes dissolved oxygen and pH in media immediately surrounding living cells cultured in a microplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells.

How long does a seahorse take to charge?

Specifications Lookah Seahorse Pro Plus Battery Capacity 650mAh Sessions Per Charge 12-15 Charge Time 1-2 Hours Charger Type USB-C15 more rows Feb 13, 2025

How long does a seahorse assay take?

The Seahorse XF Pro Analyzer and Seahorse XF Pro M cell culture microplates allow a validated performance level during an assay six hours in duration.

What does a seahorse assay tell you?

The Agilent Seahorse XF ATP Real-Time rate assay measures and quantifies the rate of ATP production from glycolytic and mitochondrial system simultaneously using label-free technology in live cells. In mammalian cells, glycolysis and oxidative phosphorylation (OXPHOS) pathways provide the majority of cellular ATP.

What is the XF glycolytic rate assay?

The XF Glycolytic Rate Assay provides a precise measure of glycolysis in live cells, revealing transient responses and rapid metabolic switches not discernible in endpoint lactate assays. Run on a Seahorse XF Analyzer, this assay quantifies proton efflux specific to glycolysis.

What drugs are in the seahorse assay?

Targets of the drugs utilized in the Seahorse assay. In the measurement of OCR, oligomycin, FCCP, and rotenone/antimycin A are sequentially utilized. Oligomycin, an inhibitor of ATP synthase, allows the measurement of ATP-linked respiration.

XF Glycolysis Stress Test Kit User Manual - Seahorse Bioscience 2025

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