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What is glycolysis stress test?

The Agilent Seahorse Glycolysis Stress Test kit measures the capacity of the glycolytic pathway after glucose starvation. This is accomplished by driving cells toward glycolysis and assessing their ability to increase glycolytic activity to meet metabolic and bioenergetic demands.

How does the Seahorse assay work?

Oxygen consumption rate (OCR) is measured before and after the addition of inhibitors to derive several parameters of mitochondrial respiration. Initially, baseline cellular OCR is measured, from which basal respiration can be derived by subtracting non-mitochondrial respiration.

What is OCR seahorse?

Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) at intervals of approximately 5-8 minutes. OCR is an indicator of mitochondrial respiration, and ECAR is largely the result of glycolysis.

What is Mito Stress Test?

The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Seahorse XFe96 extracellular flux analyzer.

What is ECAR seahorse?

Seahorse XF technology measures the flux of oxygen, the oxygen consumption rate [OCR], and the flux of protons, the extracellular acidification rate [ECAR], in the medium immediately surrounding cells in a microplate.

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