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SAMPLE SUBMISSION FORM - UCSF Mass Spectrometry Facility

SAMPLE SUBMISSION FORM - UCSF Mass Spectrometry Facility 2025

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The document is a sample submission form for the Mass Spectrometry Facility at the University of California, School of Pharmacy. It includes sections for project details, sample information, storage conditions, and requested analysis. The form requires signatures and provides guidelines for sample preparation and data submission.

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How much sample is needed for mass spectrometry?

The amount of sample required depends on the mass spectrometry service to be performed. In general, 1 mL of a 10 ppm sample or 1 mg in solid form is enough for all applications.

What is the sample concentration for mass spectrometry?

Sample concentration should be prepared with the aim to achieve a column loading of approximately 10ng, which should yield reasonable mass spectra. E.g. a concentration of approximately 0.1mg/mL would be appropriate for samples injected with a split ratio of 10:1 and a 1uL injection volume.

How do you present mass spectrometry results?

Mass spectrometry data should be given in the form: m/z 183 (M+, 41%), 168 (38), 154 (9), 138 (31) etc. The molecular ion may be specified as shown if desired. Relative intensities should be shown in parentheses (% only included once).

What is the minimum sample size for mass spectrometry?

The sensitivity of a mass spectrometer can be defined as the minimum sample size required for optimal accuracy and precision. Conventionally, 0.55 mg N are required for N isotope analysis, but recent advances in automation (see Section II.