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Isolation and Characterization of Postsynaptic Densities from Various Brain Regions Enrichment of Di

Isolation and Characterization of Postsynaptic Densities from Various Brain Regions Enrichment of Di 2025

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The document discusses the isolation and characterization of postsynaptic densities (PSDs) from various brain regions, specifically the cerebral cortex, midbrain, cerebellum, and brain stem. It highlights the morphological and protein composition differences among PSDs derived from these areas, suggesting that PSDs from the cerebrum and midbrain are associated with excitatory type I synapses, while those from the cerebellum correspond to inhibitory type II synapses. The study employs Triton X-100 for isolation and examines protein phosphorylation patterns, revealing significant variations in protein presence and activity between different brain regions. The findings contribute to understanding the functional roles of PSDs in synaptic transmission.

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What is the role of PSD?

Thus, the concept of synaptic density is used to mean the net number of surviving synapses which changes very little in adulthood, except due to the influence of neurodevelopmental abnormalities, or during some neurodegenerative disorders (Lepeta et al., 2016).

What does synaptic density mean?

At the tip of most of dendritic spines there is an electron-dense region that harbours glutamate receptors and other protein complexes, namely a postsynaptic density (PSD), which represents a postsynaptic part of a synapse4. PSD area was shown to correlate with dendritic spine volume and spine head volume5,6,7.

Which of the following brain regions is important for spatial memory?

The hippocampus and medial entorhinal cortex (MEC) are key brain areas for spatial learning and memory.

What is postsynaptic density area?

A police department that deals with complaints against officers and staff. It receives, records, investigates and resolves public complaints and also records and investigates misconduct allegations.

What is postsynaptic density region?

1) (1, 2). The postsynaptic density (PSD) is an electron-dense structure beneath the postsynaptic membrane of excitatory synapses and is usually located at the tip of the dendritic spine (Fig. 1) (24).