Request for Exemption from the NIH Guidelines for Research Involving Recombinant DNA Molecules 2025

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Researchers are responsible for immediately reporting any spill and/or accident involving recombinant or synthetic nucleic acid molecules research resulting in an overt or possible exposure, or that otherwise could lead to personal injury or illness, or to a breach of containment to their PI, the Biosafety Officer (BSO
Non-compliance may result in Suspension, limitation, or termination of financial assistance for the noncompliant NIH-funded research project and of NIH funds for other rDNA research at NAU, or The requirement for prior NIH approval of any and/or all rDNA projects at NAU.
Section III-F of the NIH Guidelines details experiments that are exempt from the requirements of the NIH Guidelines.
The steps in recombinant DNA technology include: isolating DNA from the donor and host organisms, cutting the DNA using restriction enzymes, joining the fragments with DNA ligase, introducing the recombinant DNA into the host organism, and selecting and screening transformed cells.
ing to NIH Guidelines, recombinant and synthetic nucleic acid molecules are defined as (1) molecules that a) are constructed by joining nucleic acid molecules and b) can replicate in a living cell, i.e. recombinant nucleic acids, or (2) nucleic acid molecules that are chemically or by other means synthesized or
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NIH Office of Science Policy It should be noted that only experiments covered by both III-D or III-E and III-F are considered to be exempt. If an experiment falls into Section III-A, III-B or III-C and any one of the other sections, then the rules pertaining to Sections III-A, III-B or III-C must be followed.

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