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Resolution of the light-sheet microscope 26 m, depending on the field of view) in the case of the commonly used long working distance objectives with large depth of focus. With a 10x NA 0.3 objective the axial resolution of a light-sheet microscope is better than that of a laser scanning confocal.
As the wavelength of an electron can be up to 100,000 times smaller than that of visible light, electron microscopes have a much higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes.
The Spectra 300 (S)TEM, with X-FEG/Mono or X-FEG/UltiMono, offers STEM resolution specifications of 50 pm at 300 kV, 96 pm at 60 kV and 125 pm at 30 kV with 30 pA of probe current or 100 pA with the X-CFEG. Ultra-high resolution HAADF STEM imaging on a Spectra 300 (S)TEM.
Raman microscopy applications For example, Raman microscopy can be used for quality control and failure analysis, where small defects can be selectively analyzed without damaging the sample. Of course, Raman microscopes are also used for imaging to create chemical images.
The confocal Raman imaging system alpha300 from Witec offers the unique ability to acquire chemical information non-destructively with a resolution down to the optical diffraction limit. Automated, non-destructive confocal Raman Imaging with lateral resolution of 200 - 300 nm.
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Specifications ModelVK-X3000 White light interferometry Height display resolution 0.01 nm Width display resolution 0.1 nm Surface topography repeatability 0.08 nm*4 Repeatability of RMS 0.008 nm*437 more rows

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