Section III: Cell Culture Procedures SOP-CC-001 Gelatin Coating of 2025

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Place sterilized coverslips into the wells of a 24-well plate. Add 400 L of the gelatin-coating solution, and incubate the coverslips for 10 minutes at room temperature. Remove the gelatin-coating solution, and air dry the coverslips for 15 minutes. The dried coverslips can now be stored at room temperature until use.
0.1% Gelatin Solution is used to coat cell culture flasks. Gelatin-Based Coating Solution (from bovine skin) is designed to coat cell culture flasks or dishes for 2 min and then aspirate the excess solution before seeding cells.
0.2% Gelatin Solution (GSN) is used to coat the surface of culture vessels to promote cell attachment. It is recommended for the culture of differentiated hESC cells [1, 2], as well as certain primary and immortalized cell types.
Gelatin is a protein made from animal collagen, usually from cows and pigs. Its commonly used to make capsules, cosmetics, ointments, and foods.
Gelatin is used for weight loss and for treating osteoarthritis, rheumatoid arthritis, and brittle bones (osteoporosis). Some people also use it for strengthening bones, joints, and fingernails. Gelatin is also used for improving hair condition and to shorten the recovery after exercise and sports-related injury 15.

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Gelatin is a molecular derivative of collagen and is synthesized from the alkaline digestion of collagen from bovine skin. Gelatin is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which imparts structural stability. Gelatin offers an ideal cell-attachment substrate for cell culture.
Collagen ILiquid Collagen for Cell Culture. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.

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