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Designing Custom GoldenGate Genotyping Assays - uthscsa 2025

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This technical note provides guidelines for designing and ordering custom GoldenGate Genotyping Assays using the Illumina Assay Design Tool (ADT). It outlines the process of creating custom genotyping panels, including input file types such as GeneList, RegionList, RSList, and SequenceList. The document details how to evaluate SNP loci for assay success, generate SNPScore files for ordering, and emphasizes the importance of following specific formatting requirements. The GoldenGate Assay can be utilized on BeadArray or BeadXpress platforms for high-throughput genotyping.

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