Soybean Cyst Nematode (SCN) Soil Sampling Information Sheet PSstl-scn - sdstate 2025

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Growers should take soil samples regularly for nematode testing, particularly before planting and at harvest, and should keep records of population levels in different blocks to assess the effectiveness of management programs.
Application of nematicides, pesticides which are toxic to nematodes, used to be another method for protecting soybeans from damage by SCN. These products control SCN by reducing invasion of soybean roots by juveniles of the nematode and preventing their feeding and development early in the season.
The eggs on the outside of the body hatch and juvenile nematodes re-infect soybean roots. The egg-filled body of the dead female is what is referred to as the cyst. Each cyst can contain up to 400 eggs. There can be three to four generations of SCN in a single growing season.
Soil samples for SCN can be taken anytime during the growing season if nematodes are suspected of affecting your crop. Samples can be obtained by collecting soil cores at a depth of 6-8 inches in a zig-zag pattern. While plants are actively growing in the field, take samples from within the row.
A soil test can give an estimate of the SCN population density in the soil and it can also indicate if the SCN numbers are increasing or decreasing when done periodically. To obtain a soil sample for SCN testing, collect 20 soil cores 0-6 deep in a zig-zag pattern using a soil probe or a spade.
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There are two ways to determine the presence of SCN in a field: examining soybean roots and a soil test. Scouting to determine the presence of SCN on soybean roots can be done by gently uprooting soybean plants and carefully examining the roots for female cysts (Figure 1).

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