Untitled-5 Acetamiprid (NI-25) and IC-0 Lab Method Evaluation Report No ECM0176S1-S2 - jsu 2025

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Acetamiprid is rapidly and extensively metabolized. Metabolites in urine account for 7986% of the administered dose. Only 37% of acetamiprid is collected unchanged in the urine and feces. Demethylation by phase I biotransformation is the major pathway, with 6-chloronicotinic acid being the major metabolite.
The EPA 600 methods are designed for monitoring organic pollutants in industrial and municipal waste discharges. These methods are cited under the Clean Water Act (CWA).
Acetamiprid belongs to a relatively new class of insecticide that developed in the late 1980s, the neonicotinoids. The precise structure of acetamiprid is that of a chloronicotinyl compound and it has been shown to be a potent agonist at the nicotinic acetylcholine receptors in insects.
The residues of Acetamiprid are determined by high performance liquid chromatography (HPLC) using ultraviolet (UV) detection. The limit of detection (LOD) for Acetamiprid in surface water was 0.033 ppb as established by the registrant.
Application Rates: Application rates a maximum of 0.55 pounds active ingredient per acre per season.
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