Evaluation of a Test Article in the Salmonella typhimurium - dtic 2025

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Salmonella typhimurium is a motile, aerobic to facultatively anaerobic, non-spore-forming, gram-negative bacillus and is the organism associated with enteric disease and some abortions in ruminants.
The test could be a culture that isolates the bacteria or a culture-independent diagnostic test (CIDT) that detects genetic material of the bacteria. CDC encourages laboratories to culture specimens with positive CIDT results. This process is called reflex culturing.
Infection is diagnosed when a laboratory test detects Salmonella bacteria in stool, body tissue, or fluids. The test could be a culture that isolates the bacteria or a culture-independent diagnostic test (CIDT) that detects genetic material of the bacteria.
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Key biochemical tests are fermentation of glucose, negative urease reaction, lysine decarboxylase, negative indole test, H2S production, and fermentation of dulcitol. Serological confirmation tests typically use polyvalent antisera for flagellar (H) and somatic (O) antigens.
Almost all of the identified pathogenic bacteria of humans, such as Vibrio cholerae, Pseudomonas aeruginosa, Salmonella and pathogenic E. coli, are motile. However, the ability to form biofilm is variable in human bacterial pathogens.
The AHDC performs Salmonella testing using two methods, bacterial culture (including blood cultures) and PCR (molecular testing).
The Ames test (Salmonella typhimurium reverse mutation assay) is a bacterial short-term test for identification of carcinogens using mutagenicity in bacteria as an end point.

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