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How do you calculate matrix spike recovery?

Dilute the sample and diluent aliquots 1:2 (50%) with 100 L of the 1:2 calibrator series. Calculate the %Recovery for each spiked aliquot: %Recovery = ((Observed Concentration Endogenous Concentration)/ Spiked Diluent Concentration)*100.

How do you spike a water sample?

For most analytical schedules, concentrations of the spike solutions are designed to provide  0.1 microgram per liter (g/L) of each analyte in a water sample by injecting 100 L of the spike solution into a 1-L filtered water sample.

What is the spike method in analysis?

Answer and Explanation: Spiking in analytical chemistry refers to the act of adding a known amount of a known substance to a sample in order to evaluate the performance of an analytical method.

How do you spike a solution?

Use a spiking solution that is about 50 to 100 times higher than the chosen spiking level. 3. Determine the amount of spiking solution to be added to the sample as follows: Volume of Spiking Solution = Spike concentration desired x Volume of sample to which spike is added Concentration of the spiking solution 4.