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INSTRUCTIONS FOR FIELD USE OF SPIKE 5 3 2 SOLUTIONS - water usgs

INSTRUCTIONS FOR FIELD USE OF SPIKE 5 3 2 SOLUTIONS - water usgs 2025

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The document provides detailed instructions for the field use of spike solutions in organic-analyte water samples, emphasizing the importance of matrix spikes for quality control. It explains how matrix spikes help assess the impact of sample matrices on analytical methods and outlines procedures for preparing and using field and laboratory matrix spikes. The document also includes safety precautions, equipment requirements, and guidelines for sample coding, disposal of materials, and shipping protocols to ensure accurate analysis and minimize degradation of organic analytes.

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What is the spiking method?

Standard addition or spiking is an analytical chemistry technique used to enhance the measurement of weak signals by techniques such as atomic absorption spectroscopy (AAS) and gas chromatography (GS).

What does spike mean in chemistry?

isotopically enriched sample, called a spike, is added to the original material, thoroughly mixed with it, and extracted with that element.

What is the spike recovery procedure?

In spike and recovery, a known amount of analyte is added (spiked) into the natural test sample matrix. Then the assay (here assumed to be ELISA) is run to measure the response (recovery) of the spiked sample matrix compared to an identical spike in the standard diluent.

How do you spike a solution?

Use a spiking solution that is about 50 to 100 times higher than the chosen spiking level. 3. Determine the amount of spiking solution to be added to the sample as follows: Volume of Spiking Solution = Spike concentration desired x Volume of sample to which spike is added Concentration of the spiking solution 4.

What does it mean to spike a solution?

A Spiking Solution is a standard that is chosen for preparing a matrix spike; the concentration of the analyte in the spiking solution is usually 50 -100 times higher than the concentration found in the unspiked sample.

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What is the purpose of a spike solution?

A spike solution is a very simple program to explore potential solutions. Build the spike to only addresses the problem under examination and ignore all other concerns. Most spikes are not good enough to keep, so expect to throw it away.

Why do we spike samples?

In general, spiked standards offer advantages of low cost of preparation, of advance assurance that the amount and chemical form of the radioisotopes added are known, and of the possibility of preparation of unnatural matrices, or of nuclides that are to be expected but not yet to be found in nature.

How do you spike a water sample?

For most analytical schedules, concentrations of the spike solutions are designed to provide  0.1 microgram per liter (g/L) of each analyte in a water sample by injecting 100 L of the spike solution into a 1-L filtered water sample.

INSTRUCTIONS FOR FIELD USE OF SPIKE 5 3 2 SOLUTIONS - water usgs 2025

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